James Arnold Taylor

as Leonardo (voice)

Mitchell Whitfield

as Donatello / Additional Voices (voice)

Nolan North

as Raphael / Nightwatcher (voice)

Mikey Kelley

as Michelangelo (voice)

Sarah Michelle Gellar

as April O'Neil (voice)

Chris Evans

as Casey Jones (voice)

Mako

as Splinter (voice)

Patrick Stewart

as Winters (voice)

Zhang Ziyi

as Karai (voice)

Kevin Smith

as Diner Cook (voice)

Laurence Fishburne

as Narrator (voice)

John DiMaggio

as Colonel Santino (voice)

Paula Mattioli

as General Serpiente (voice)

Kevin Michael Richardson

as General Aguila / Additional Voices (voice)

Fred Tatasciore

as General Gato / Additional Voices (voice)

Dee Bradley Baker

as Additional Voices (voice)

Greg Baldwin

as Splinter (voice)

Jeff Bennett

as Additional Voices (voice)

Jim Cummings

as Additional Voices (voice)

Grey DeLisle

as Additional Voices (voice)

Chris Edgerly

as Additional Voices (voice)

Kim Mai Guest

as Additional Voices (voice)

Jennifer Hale

as Additional Voices (voice)

Jess Harnell

as Additional Voices (voice)

Phil LaMarr

as Additional Voices (voice)

Paul Michael Robinson

as Additional Voices (voice)

Tara Strong

as Additional Voices (voice)

Billy West

as Additional Voices (voice)

Kevin Munroe

as Diner Patron (voice) (uncredited)

Frank Welker

as General Mono (voice) (uncredited)