Ron Perlman

as Hellboy (voice)

Selma Blair

as Liz Sherman (voice)

Doug Jones

as Abe Sapien (voice)

John Hurt

as Professor Trevor 'Broom' Bruttenholm (voice)

Peri Gilpin

as Professor Kate Corrigan (voice)

J. Grant Albrecht

as Oliver Trombolt (voice)

Jim Cummings

as Tom Manning (voice)

Grey DeLisle

as Anna / Harpy-Hag #2 (voice)

Rob Paulsen

as Sydney Leach / Anna's Fiance (voice)

Kath Soucie

as Erzsebet Ondrushko (voice)

DeeDee Rescher

as Harpy-Hag #1 (voice)

Cree Summer

as Hecate (voice)

James Arnold Taylor

as Young Broom / Father Lupescu (voice)