Kate Higgins

as Frankie Stein / Lilith Van Helscream (voice)

Cindy Robinson

as Jackson Jekyll / Holt Hyde / Operetta / Additional Voice (voice)

Salli Saffioti

as Clawdeen Wolf / Cleo de Nile (voice)

Erin Fitzgerald

as Abbey Bominable / C.A. Cupid / Clair / Additional Voices (voice)

Laura Bailey

as Lagoona Blue / Headmistress Bloodgood (voice)

Debi Derryberry

as Draculaura (voice)

Ogie Banks

as Clawd Wolf (voice)

Evan Smith

as Deuce Gorgon / Gil Webber / Thad (voice)

America Young

as Howleen Wolf / Toralei Stripe (voice)

Audu Paden

as Ghoulia Yelps / Manny Taur / Sheriff / Additional voices (voice)

Cam Clarke

as Heath Burns / Mr. Rotter / Goon #2 (voice)

Julie Maddalena

as Robecca Steam / Venus McFlytrap (voice)

Steve Staley

as Chad / Kid #1 (voice)

Mark Mercado

as Gargoyle (voice)

Ira Singerman

as Goon #1 (voice)

Andrew Duncan

as Narrator (voice)

Wendee Lee

as Nefera De Nile (voice) (uncredited)

Jonathan Lipow

as Tiki People (voice) (uncredited)