Ivan Trojan

as Myšpulín (voice)

Ondřej Gregor Brzobohatý

as Piňďa (voice)

Tereza Bebarová

as Fifinka (voice)

Bohdan Tůma

as Bobík (voice)

Václav Postránecký

as Rudolf II. (voice)

Vladimír Brabec

as Sendivoj (voice)

Jan Přeučil

as Zimozel (voice)

Jiří Lábus

as kouzelník Kelley (voice)

Miroslav Táborský

as komoří (voice)

Petr Rychlý

as Tycho de Brahe / náčelník (voice)

Anna Brousková

as Reportérka Káťa Hvozdíková (voice)

Vladimír Javorský

as Primář (voice)

Lucie Bílá

as sestřička (voice)

Gabriela Osvaldová

as Dr. Malebná (voice)

Zlata Adamovská

as magistra Stříbrná (voice)

Martin Písařík

as Tonda (voice)

Tomáš Juřička

as Franta (voice)

Kryštof Hádek

as Lojzík (voice)

Martha Issová

as pošťačka (voice)

Ota Jirák

as lev Aurix (voice)