Frank Welker

as Fred Jones / Scooby-Doo (voice)

Casey Kasem

as Shaggy Rogers (voice)

Heather North

as Daphne Blake (voice)

Nicole Jaffe

as Velma Dinkley (voice)

Candi Milo

as Charlene Otero / Museum Guide / Old Woman #1 (voice)

Maria Canals-Barrera

as Sofia Otero / Old Woman #2 (voice)

Jesse Borrego

as Luis Otero / Musician #2 / Suspicious Tourist (voice)

Brandon Gonzales

as Jorge Otero (voice)

Castulo Guerra

as Señor Fuente / Man #1 (voice)

Benito Martinez

as El Curandero / Man #2 (voice)

Darlene Mendoza

as Natalia Otero / Little Girl (voice)

Rita Moreno

as Doña Dolores / Woman #3 (voice)

Allyse Rivera

as Carolina Otero (voice)

Casey Sandino

as Sebastian Otero / Little Boy (voice)

Eddie Santiago

as Alejo Otero / Musician #1 (voice)

Rip Taylor

as Mr. Smiley / Ghost of Señor Otero (voice)

Michael Forest

as El Chuprecabra (voice)