Kevin Conroy

as Batman / Bruce Wayne (voice)

Neal McDonough

as Deadshot / Floyd Lawton (voice)

Hynden Walch

as Harley Quinn / Harleen Quinzel (voice)

Matthew Gray Gubler

as The Riddler / Edward Nigma (voice)

Troy Baker

as The Joker (voice)

Eric Bauza

as Security Guy (voice)

Chris Cox

as G.C.P.D. Commissioner James Gordon (voice)

John DiMaggio

as King Shark / Nanaue / Bane (voice)

Greg Ellis

as Captain Boomerang (voice)

Giancarlo Esposito

as Black Spider / Eric Needham (voice)

Jennifer Hale

as Killer Frost / Louise Lincoln (voice)

Martin Jarvis

as Alfred Pennyworth (voice)

Peter Jessop

as Watch Commander (voice)

Christian Lanz

as Scarecrow / Dr. Jonathan Crane / Victor Zsasz (voice)

Nolan North

as The Penguin / Oswald Cobblepot / KGBeast / Anatoli Knyazev (voice)

Christopher Lee Parson

as Stout Guard (voice)

CCH Pounder

as Amanda Waller (voice)

Andrea Romano

as Woman (voice)

Travis Willingham

as Morgue Guy / Penguin Thug (voice)

Mick Wingert

as Joker Security Guy (voice)