Ed Asner

as J. Jonah Jameson (voice)

Dorian Harewood

as Tombstone (voice)

Christopher Daniel Barnes

as Spider-Man/Peter Parker (voice)

Sara Ballantine

as Mary Jane Watson (voice)

Leigh-Allyn Baker

as Alisha Silvermain (voice)

Maxwell Caulfield

as Alistair Smythe (voice)

Linda Gary

as Tante May Parker (voice)

Gary Imhoff

as Harry Osborn (voice)

Roscoe Lee Browne

as Kingpin/Wilson Fisk (voice)