Jean Dujardin

as Lucky Luke

Melvil Poupaud

as Jesse James

Sylvie Testud

as Calamity Jane

Alexandra Lamy

as Belle

Michaël Youn

as Billy The Kid

Daniel Prévost

as Pat Poker

Chloé Jouannet

as Eleanor

Jean-François Balmer

as Le gouverneur

André Oumansky

as President

Gabriel Corrado

as Luke's Father

Pompeyo Audivert

as Undertaker

Atilio Pozzobón

as Barman

Alberto Laiseca

as Dick Digger

Mathias Sandor

as Young Luke

Carolina Presno

as Luke's Mother

Carlos Kaspar

as Hank

Mirta Wons

as Mrs. Flimsey

Jorge Noya

as Mr. Flimsey

Claudio Weppler

as Phil Defer

Horacio Marassi

as Colonel Mc Straggle

Daniel Campomenosi

as Dr. Doxey

Kazuomi Takagi

as Ming Li Fu

Luciano Cáceres

as Henchman

Joaquín Berthold

as Henchman

Martín Kohan

as Henchman

Óscar Kramer

as Politician

Daniela Catz

as Saloon Girl

Bruno Salomone

as Jolly Jumper (voice)