Bruce Greenwood

as Batman / Bruce Wayne (voice)

Jensen Ackles

as Red Hood / Jason Todd (voice)

Neil Patrick Harris

as Nightwing (voice)

Jason Isaacs

as Ra's al Ghul (voice)

John DiMaggio

as Joker (voice)

Wade Williams

as Black Mask (voice)

Carlos Alazraqui

as Chi Chi / Thug #1 / Baton (voice)

Robert Clotworthy

as Leon / Thug (voice)

Gary Cole

as Bobo / Commissioner Gordon / Shot / Guard (voice)

Brian George

as Ra's Assistant (voice)

Kelly Hu

as Ms. Li (voice)

Phil LaMarr

as Rick / Bulk (voice)

Alexander Martella

as Young Robin / Jason Todd (voice)

Vincent Martella

as Robin / Jason Todd (voice)

Jim Piddock

as Alfred (voice)

Kevin Michael Richardson

as Tyler Bramford (voice)

Andrea Romano

as Reporter #1 (voice)

Dwight Schultz

as Freddie / Drug Dealer (voice)

Fred Tatasciore

as Mercenary #1 / Amazo / Guard (voice)

Keri Tombazian

as Reporter #2 (voice)

Bruce Timm

as The Riddler (voice)

Michael Villani

as Reporter #3 (voice)