Michael York

as Sherlock Holmes (voice)

Malcolm McDowell

as Professor Moriarity (voice)

John Rhys-Davies

as Dr. Watson (voice)

Billy West

as Tom (voice)

Grey DeLisle

as Red (voice)

Phil LaMarr

as Spike / Policeman (voice)

Kath Soucie

as Tuffy (voice)

Jeff Bergman

as Butch / Droopy (voice)

Greg Ellis

as Tin / Sergeant (voice)

Jess Harnell

as Pan / Brett Jeremy (voice)

Richard McGonagle

as Ali / First Policeman (voice)