Bruce Greenwood

as Bruce Wayne / Batman (voice)

Jennifer Carpenter

as Selina Kyle (voice)

Anthony Stewart Head

as Alfred (voice)

Scott Patterson

as James Gordon (voice)

John DiMaggio

as Chief Bullock / Big Bill Dust / Additional Voices (voice)

Yuri Lowenthal

as Harvey Dent (voice)

Grey DeLisle

as Sister Leslie / Jason / Additional Voices (voice)

William Salyers

as Hugo Strange (voice)

Bob Joles

as Mayor Tolliver (voice)

Tara Strong

as Marlene (voice)

Kari Wührer

as Barbara Gordon / Pamela Isley (voice)

Lincoln Melcher

as Dickie (voice)

Bruce Timm

as Arkham Radio Man (voice)

David Forseth

as Cyrus Gold (voice)

Chris Cox

as Father Callahan / Additional Voices (voice)