Troy Baker

as Batman / Joker (voice)

Eric Bauza

as Leonardo (voice)

Darren Criss

as Raphael (voice)

Kyle Mooney

as Michelangelo (voice)

Baron Vaughn

as Donatello (voice)

Carlos Alazraqui

as Bane (voice)

Cas Anvar

as Ra's al Ghul (voice)

Rachel Bloom

as Batgirl (voice)

John DiMaggio

as Mr. Freeze (voice)

Keith Ferguson

as Baxter Stockman / Two-Face (voice)

Brian George

as Alfred (voice)

Ben Giroux

as Robin (voice)

Tom Kenny

as Penguin (voice)

Andrew Kishino

as Shredder (voice)

Jim Meskimen

as Commissioner Gordon / Scarecrow (voice)

Tara Strong

as Harley Quinn / Poison Ivy (voice)