Frank Welker

as Scooby-Doo / Fred Jones / Msicellaneous Bikers (voice)

Mindy Cohn

as Velma Dinkley (voice)

Grey DeLisle

as Daphne Blake (voice)

Matthew Lillard

as Shaggy Rogers / Shaky Joe (voice)

Cathy Cavadini

as Faith (voice)

John DiMaggio

as Friz / GPS / Msicellaneous Bikers (voice)

Michael Gough

as Mr. Babbit / Blair / Grad Student #1 / Bikers (voice)

Matthew Gray Gubler

as Winsor (voice)

Finola Hughes

as Professor Svankmajer (voice)

Maulik Pancholy

as Doctor (voice)

Kevin Michael Richardson

as Tex / Cop #2 / Grad Student #3 / Bikers (voice)

Fred Willard

as Mr. Hubley (voice)

Dave Wittenberg

as Policeman / Cop #1 / Grad Student #2 / Bikers (voice)

Gwendoline Yeo

as Ms. Deitch (voice)