Tomokazu Sugita

as Sakata Gintoki (voice)

Daisuke Sakaguchi

as Shimura Shinpachi (voice)

Rie Kugimiya

as Kagura (voice)

Akira Ishida

as Katsura Kotarou (voice)

Takehito Koyasu

as Takasugi Shinsuke (voice)

Yutaka Aoyama

as Okada Nizou (voice)

Takeharu Oonishi

as Murata Tetsuya (voice)

Keiko Nemoto

as Murata Tetsuko (voice)

Chafurin

as Henpeita Takeshi (voice)

Risa Hayamizu

as Kijima Matako (voice)

Kiyonobu Suzuki

as Murata Jintetsu (voice)

Satsuki Yukino

as Shimura Tae (voice)

Mikako Takahashi

as Sadaharu (voice)

Susumu Chiba

as Kondou Isao (voice)

Kazuya Nakai

as Hijikata Toushirou (voice)

Kenichi Suzumura

as Okita Sougo (voice)

Tetsuharu Ota

as Yamazaki Sagaru (voice)

Takumi Yamazaki

as Kawakami Bansai (voice)

Hochu Otsuka

as Abuto (voice)

Satoshi Hino

as Kamui (voice)

Sumi Shimamoto

as Okita Mitsuba (voice)

Kujira

as Terada Ayano (voice)

Yuu Sugimoto

as Catherine (voice)

Yu Kobayashi

as Sarutobi Ayame (voice)

Fumiko Orikasa

as Yagyuu Kyuubee (voice)

Koji Yusa

as Toujou Ayumu (voice)

Fumihiko Tachiki

as Hasegawa Taizou (voice)

Shin-ichiro Miki

as Sakamoto Tatsuma (voice)

Norio Wakamoto

as Matsudaira Katakuriko (voice)

Kouichi Sakaguchi

as Prince Hata (voice)

Shinji Takamatsu

as Elizabeth (voice)

Koichi Yamadera

as Yoshida Shouyou (voice)

Shigeo Kiyama

as Additional Voices

Tsutomu Densaka

as Additional Voices

Masahiro Okazaki

as Additional Voices