James Urbaniak

as Dr. Venture / Phantom Limb / Jonas Venture Jr. (voice)

Patrick Warburton

as Brock Samson (voice)

Michael Sinterniklaas

as Dean Venture (voice)

Doc Hammer

as Dr. Mrs. The Monarch / Henchman 21 / Shoreleave / Billy Quizboy / Dermott / Red Mantle / Rose / Ward (voice)

Christopher McCulloch

as Hank Venture / The Monarch / Sgt. Hatred / Hunter Gathers / Pete White / Watch (voice)

Nina Arianda

as Mantilla (voice)

Clancy Brown

as Red Death / Daisy (voice)

John Hodgman

as Snoopy (voice)

Hal Lublin

as Clayton / Seth / Tour Guide (voice)

Jane Lynch

as Bobbi St. Simone (voice)

Charles Parnell

as Jefferson Twilight / OSI Agent (voice)

Jay Pharoah

as Nuno Blood / Additional Blackulas (voice)

Steven Rattazzi

as Dr. Orpheus (voice)

J.K. Simmons

as Ben (voice)

Dana Snyder

as The Alchemist / Conductor / Houseless Guy (voice)