Dakota Fanning

as Fern Arable

Julia Roberts

as Charlotte the Spider (voice)

Steve Buscemi

as Templeton the Rat (voice)

John Cleese

as Samuel the Sheep (voice)

Oprah Winfrey

as Gussy the Goose (voice)

Cedric the Entertainer

as Golly the Goose (voice)

Reba McEntire

as Betsy the Cow (voice)

Kathy Bates

as Bitsy the Cow (voice)

Robert Redford

as Ike the Horse (voice)

Thomas Haden Church

as Brooks the Crow (voice)

André 3000

as Elwyn the Crow (voice)

Dominic Scott Kay

as Wilbur (voice)

Sam Shepard

as Narrator (voice)

Abraham Benrubi

as Uncle the Pig (voice)

Kevin Anderson

as Mr. Arable

Essie Davis

as Mrs. Arable

Siobhan Fallon Hogan

as Mrs. Zuckerman

Louis Corbett

as Avery

Robyn Arthur

as Teacher

Julian O'Donnell

as Henry Fussy

Gary Basaraba

as Homer Zuckerman

Nate Mooney

as Lurvy

Nicholas Bell

as Minister

Beau Bridges

as Dr. Dorian

Teague Rook

as Well Dressed Man

Julia Zemiro

as Bystander

Denise Kirby

as New Teacher

Robert Plazek

as Ball Thrower

Joseph Lotesto

as Young Boy

Michael Roland

as Mayor

Don Bridges

as Announcer

Ian Watkin

as Fair Official

Joel McCrary

as Sheep Group (voice)

Brian Stepanek

as Sheep Group (voice)

Fred Tatasciore

as Sheep Group (voice)

Bradley White

as Sheep Group (voice)

Maia Kirkpatrick

as Joy the Baby Spider (voice)

Jennessa Rose

as Aranea the Baby Spider (voice)

Briana Hodge

as Nellie the Baby Spider (voice)

Dale Azzopardi

as Photographer (uncredited)

Ella Scott Lynch

as Girl at Fair (uncredited)

Abraham Benrubi

as Uncle The Fatter Pig(voice)

Joel McCrary

as Sheep Group

Brian Stepanek

as Sheep Group

Fred Tatasciore

as Sheep Group